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New updates include:
- AutoSpill compensation: AutoSpill is a new algorithm for calculating spillover and producing a compensation matrix developed and incorporated into FlowJo v10.7 in collaboration with researchers from the Vlaams Instituut voor Biotechnologie in Belgium and the the Babraham Institute in Cambridge, UK.
- Our exclusive AutoSpread algorithm provides a Spillover Spreading Matrix (SSM) by calculating spillover spreading coefficients with linear models, removing the need for well-defined positive and negative populations in single-color controls.
'Together, AutoSpill and AutoSpread remove limiting constraints of traditional compensation methods, easing the preparation of compensation controls in high-parameter flow cytometry, making errors less likely, and facilitating the practical implementation of ultra high-parameter flow cytometry.' —Roca, et al
Read the full article
AutoSpill: a method for calculating spillover coefficients in high-parameter flow cytometry.
Authors: Carlos P. Roca, Oliver T. Burton, Teresa Prezzemolo, Carly E. Whyte, Richard Halpert, Łukasz Kreft, James Collier, Alexander Botzki, Josef Spidlen, Stéphanie Humblet-Baron, Adrian Liston. - Proliferation and Cell Cycle models have been updated to better fit data with missing peaks.
- The t-SNE derived parameters now remain connected to workspace files after saving. Note: In case derivative files are ever dissociated from a workspace, drag and drop the CSV back onto the data file or right click and choose “Import Parameters from CSV/CLR”.
- Figures added to the Layout Editor will no longer lose parameter annotations without user deselecting those features manually or setting them hidden in the preferences.
- Total Spread Matrix (TSM) is now available for matrices created in FlowJo. TSM is the Spillover Spreading Matrix (SSM) without normalization to the florescent intensity of the probe.
For more details on features in this release, and a comprehensive list of known issues visit the exhaustive release notes.
FlowJo macOS users: please read this update on Big Sur
